Software:Article Title: The molecular architecture of myelinated peripheral nerves is supported by calorie restriction with aging
Article Snippet: To determine statistical significance between samples, unpaired t-test was performed using GraphPad Prism v5.0 software.Graph pad Prism software. table ft1 table-wrap mode="anchored" t5 caption a7 Species Antigen Source and Catalog # Dilution WB IS Chicken P0 Gift from Dr.Gerry Shaw, UF 1:10000 1:500 Rabbit PMP22 Pareek et. al, 1997; n/a 1:1000 n/a Rat MBP Chemicon, Temecula; MAB386 1:5000 n/a Mouse GAPDH Encor Biotechnology Inc.; MCA-1D4 1:5000 n/a Mouse NF-H Encor Biotechnology Inc.; MCA-NAP4 1:500 n/a Chicken NF-M Encor Biotechnology Inc.; CPCA-NF-M 1:5000 n/a Chicken NF-L Encor Biotechnology Inc.; CPCA-NF-L 1:5000 1:1000 Mouse Vimentin Sigma, St Louis, MO, USA; clone VIM 13.2 1:1000 1:500 Chicken MBP Encor Biotechnology, Inc.; CPCA-MBP n/a 1:500 Rabbit p75 NTR Chemicon; AB1554 1:10000 n/a Mouse pHH3 (Ser10) Millipore, Temecula; 05–598 1:500 1:200 Rabbit Pan-Na v (α-subunit) Chemicon; AB5210 1:250 1:100 Rabbit Kv1.1 Chemicon; AB5174 1:250 1:100 Rabbit HSF1 Stressgen; Victoria British Columbia; SPA- 901 1:500 1:250 Rat HSP90 Stressgen, SPA-835 1:2000 n/a Rabbit HSP70 Stressgen; SPA-812 1:1000 1:500 Rabbit HSP40 Stressgen; SPA-400 1:2000 n/a Goat HSP27 Santa Cruz Biotechnology, Inc.; sc-1049 1:2000 n/a Rabbit αB-crystallin Stressgen; SPA-223 1:1000 n/a Rabbit LAMP1 Gift from Dr. William Dunn Jr, UF 1:2000 n/a Rabbit Atg7 Gift from Dr. William Dunn Jr, UF 1:3000 n/a Rabbit pS6 (Ser235/236) Cell Signaling Technology, Inc., 2211 1:800 n/a Mouse S6 Cell Signaling Technology, Inc.; 2317 1:800 n/a Mouse HSP70 Stressgen; SPA-810 1:1000 1:250 Mouse LAMP1 Stressgen; VAM-EN001 n/a 1:250 Rabbit Cathepsin-D Cortex Biochem, San Leanardo, CA, USA; CP3090 1:1000 n/a Rabbit LC3 Cell Signaling Technology; 2775 1:1000 1:200 Open in a separate window WB: Western Blot; IS: Immunostaining; n/a: non-applicable Primary antibodies used in this study
Article Title: The molecular architecture of myelinated peripheral nerves is supported by calorie restriction with aging
Article Snippet: To determine statistical significance between samples, unpaired t-test was performed using GraphPad Prism v5.0 software.Graph pad Prism software. table ft1 table-wrap mode="anchored" t5 caption a7 Species Antigen Source and Catalog # Dilution WB IS Chicken P0 Gift from Dr.Gerry Shaw, UF 1:10000 1:500 Rabbit PMP22 Pareek et. al, 1997; n/a 1:1000 n/a Rat MBP Chemicon, Temecula; MAB386 1:5000 n/a Mouse GAPDH Encor Biotechnology Inc.; MCA-1D4 1:5000 n/a Mouse NF-H Encor Biotechnology Inc.; MCA-NAP4 1:500 n/a Chicken NF-M Encor Biotechnology Inc.; CPCA-NF-M 1:5000 n/a Chicken NF-L Encor Biotechnology Inc.; CPCA-NF-L 1:5000 1:1000 Mouse Vimentin Sigma, St Louis, MO, USA; clone VIM 13.2 1:1000 1:500 Chicken MBP Encor Biotechnology, Inc.; CPCA-MBP n/a 1:500 Rabbit p75 NTR Chemicon; AB1554 1:10000 n/a Mouse pHH3 (Ser10) Millipore, Temecula; 05–598 1:500 1:200 Rabbit Pan-Na v (α-subunit) Chemicon; AB5210 1:250 1:100 Rabbit Kv1.1 Chemicon; AB5174 1:250 1:100 Rabbit HSF1 Stressgen; Victoria British Columbia; SPA- 901 1:500 1:250 Rat HSP90 Stressgen, SPA-835 1:2000 n/a Rabbit HSP70 Stressgen; SPA-812 1:1000 1:500 Rabbit HSP40 Stressgen; SPA-400 1:2000 n/a Goat HSP27 Santa Cruz Biotechnology, Inc.; sc-1049 1:2000 n/a Rabbit αB-crystallin Stressgen; SPA-223 1:1000 n/a Rabbit LAMP1 Gift from Dr. William Dunn Jr, UF 1:2000 n/a Rabbit Atg7 Gift from Dr. William Dunn Jr, UF 1:3000 n/a Rabbit pS6 (Ser235/236) Cell Signaling Technology, Inc., 2211 1:800 n/a Mouse S6 Cell Signaling Technology, Inc.; 2317 1:800 n/a Mouse HSP70 Stressgen; SPA-810 1:1000 1:250 Mouse LAMP1 Stressgen; VAM-EN001 n/a 1:250 Rabbit Cathepsin-D Cortex Biochem, San Leanardo, CA, USA; CP3090 1:1000 n/a Rabbit LC3 Cell Signaling Technology; 2775 1:1000 1:200 Open in a separate window WB: Western Blot; IS: Immunostaining; n/a: non-applicable Primary antibodies used in this study Immunochemistry Schwann cells on glass coverslips were fixed with 4 % paraformaldehyde (10 min) and permeabilized with ice-cold 100% methanol (5 min).
Western Blot:Article Title: The molecular architecture of myelinated peripheral nerves is supported by calorie restriction with aging
Article Snippet: To determine statistical significance between samples, unpaired t-test was performed using GraphPad Prism v5.0 software.Graph pad Prism software. table ft1 table-wrap mode="anchored" t5 caption a7 Species Antigen Source and Catalog # Dilution WB IS Chicken P0 Gift from Dr.Gerry Shaw, UF 1:10000 1:500 Rabbit PMP22 Pareek et. al, 1997; n/a 1:1000 n/a Rat MBP Chemicon, Temecula; MAB386 1:5000 n/a Mouse GAPDH Encor Biotechnology Inc.; MCA-1D4 1:5000 n/a Mouse NF-H Encor Biotechnology Inc.; MCA-NAP4 1:500 n/a Chicken NF-M Encor Biotechnology Inc.; CPCA-NF-M 1:5000 n/a Chicken NF-L Encor Biotechnology Inc.; CPCA-NF-L 1:5000 1:1000 Mouse Vimentin Sigma, St Louis, MO, USA; clone VIM 13.2 1:1000 1:500 Chicken MBP Encor Biotechnology, Inc.; CPCA-MBP n/a 1:500 Rabbit p75 NTR Chemicon; AB1554 1:10000 n/a Mouse pHH3 (Ser10) Millipore, Temecula; 05–598 1:500 1:200 Rabbit Pan-Na v (α-subunit) Chemicon; AB5210 1:250 1:100 Rabbit Kv1.1 Chemicon; AB5174 1:250 1:100 Rabbit HSF1 Stressgen; Victoria British Columbia; SPA- 901 1:500 1:250 Rat HSP90 Stressgen, SPA-835 1:2000 n/a Rabbit HSP70 Stressgen; SPA-812 1:1000 1:500 Rabbit HSP40 Stressgen; SPA-400 1:2000 n/a Goat HSP27 Santa Cruz Biotechnology, Inc.; sc-1049 1:2000 n/a Rabbit αB-crystallin Stressgen; SPA-223 1:1000 n/a Rabbit LAMP1 Gift from Dr. William Dunn Jr, UF 1:2000 n/a Rabbit Atg7 Gift from Dr. William Dunn Jr, UF 1:3000 n/a Rabbit pS6 (Ser235/236) Cell Signaling Technology, Inc., 2211 1:800 n/a Mouse S6 Cell Signaling Technology, Inc.; 2317 1:800 n/a Mouse HSP70 Stressgen; SPA-810 1:1000 1:250 Mouse LAMP1 Stressgen; VAM-EN001 n/a 1:250 Rabbit Cathepsin-D Cortex Biochem, San Leanardo, CA, USA; CP3090 1:1000 n/a Rabbit LC3 Cell Signaling Technology; 2775 1:1000 1:200 Open in a separate window WB: Western Blot; IS: Immunostaining; n/a: non-applicable Primary antibodies used in this study
Article Title: The molecular architecture of myelinated peripheral nerves is supported by calorie restriction with aging
Article Snippet: To determine statistical significance between samples, unpaired t-test was performed using GraphPad Prism v5.0 software.Graph pad Prism software. table ft1 table-wrap mode="anchored" t5 caption a7 Species Antigen Source and Catalog # Dilution WB IS Chicken P0 Gift from Dr.Gerry Shaw, UF 1:10000 1:500 Rabbit PMP22 Pareek et. al, 1997; n/a 1:1000 n/a Rat MBP Chemicon, Temecula; MAB386 1:5000 n/a Mouse GAPDH Encor Biotechnology Inc.; MCA-1D4 1:5000 n/a Mouse NF-H Encor Biotechnology Inc.; MCA-NAP4 1:500 n/a Chicken NF-M Encor Biotechnology Inc.; CPCA-NF-M 1:5000 n/a Chicken NF-L Encor Biotechnology Inc.; CPCA-NF-L 1:5000 1:1000 Mouse Vimentin Sigma, St Louis, MO, USA; clone VIM 13.2 1:1000 1:500 Chicken MBP Encor Biotechnology, Inc.; CPCA-MBP n/a 1:500 Rabbit p75 NTR Chemicon; AB1554 1:10000 n/a Mouse pHH3 (Ser10) Millipore, Temecula; 05–598 1:500 1:200 Rabbit Pan-Na v (α-subunit) Chemicon; AB5210 1:250 1:100 Rabbit Kv1.1 Chemicon; AB5174 1:250 1:100 Rabbit HSF1 Stressgen; Victoria British Columbia; SPA- 901 1:500 1:250 Rat HSP90 Stressgen, SPA-835 1:2000 n/a Rabbit HSP70 Stressgen; SPA-812 1:1000 1:500 Rabbit HSP40 Stressgen; SPA-400 1:2000 n/a Goat HSP27 Santa Cruz Biotechnology, Inc.; sc-1049 1:2000 n/a Rabbit αB-crystallin Stressgen; SPA-223 1:1000 n/a Rabbit LAMP1 Gift from Dr. William Dunn Jr, UF 1:2000 n/a Rabbit Atg7 Gift from Dr. William Dunn Jr, UF 1:3000 n/a Rabbit pS6 (Ser235/236) Cell Signaling Technology, Inc., 2211 1:800 n/a Mouse S6 Cell Signaling Technology, Inc.; 2317 1:800 n/a Mouse HSP70 Stressgen; SPA-810 1:1000 1:250 Mouse LAMP1 Stressgen; VAM-EN001 n/a 1:250 Rabbit Cathepsin-D Cortex Biochem, San Leanardo, CA, USA; CP3090 1:1000 n/a Rabbit LC3 Cell Signaling Technology; 2775 1:1000 1:200 Open in a separate window WB: Western Blot; IS: Immunostaining; n/a: non-applicable Primary antibodies used in this study Immunochemistry Schwann cells on glass coverslips were fixed with 4 % paraformaldehyde (10 min) and permeabilized with ice-cold 100% methanol (5 min).
Immunostaining:Article Title: The molecular architecture of myelinated peripheral nerves is supported by calorie restriction with aging
Article Snippet: To determine statistical significance between samples, unpaired t-test was performed using GraphPad Prism v5.0 software.Graph pad Prism software. table ft1 table-wrap mode="anchored" t5 caption a7 Species Antigen Source and Catalog # Dilution WB IS Chicken P0 Gift from Dr.Gerry Shaw, UF 1:10000 1:500 Rabbit PMP22 Pareek et. al, 1997; n/a 1:1000 n/a Rat MBP Chemicon, Temecula; MAB386 1:5000 n/a Mouse GAPDH Encor Biotechnology Inc.; MCA-1D4 1:5000 n/a Mouse NF-H Encor Biotechnology Inc.; MCA-NAP4 1:500 n/a Chicken NF-M Encor Biotechnology Inc.; CPCA-NF-M 1:5000 n/a Chicken NF-L Encor Biotechnology Inc.; CPCA-NF-L 1:5000 1:1000 Mouse Vimentin Sigma, St Louis, MO, USA; clone VIM 13.2 1:1000 1:500 Chicken MBP Encor Biotechnology, Inc.; CPCA-MBP n/a 1:500 Rabbit p75 NTR Chemicon; AB1554 1:10000 n/a Mouse pHH3 (Ser10) Millipore, Temecula; 05–598 1:500 1:200 Rabbit Pan-Na v (α-subunit) Chemicon; AB5210 1:250 1:100 Rabbit Kv1.1 Chemicon; AB5174 1:250 1:100 Rabbit HSF1 Stressgen; Victoria British Columbia; SPA- 901 1:500 1:250 Rat HSP90 Stressgen, SPA-835 1:2000 n/a Rabbit HSP70 Stressgen; SPA-812 1:1000 1:500 Rabbit HSP40 Stressgen; SPA-400 1:2000 n/a Goat HSP27 Santa Cruz Biotechnology, Inc.; sc-1049 1:2000 n/a Rabbit αB-crystallin Stressgen; SPA-223 1:1000 n/a Rabbit LAMP1 Gift from Dr. William Dunn Jr, UF 1:2000 n/a Rabbit Atg7 Gift from Dr. William Dunn Jr, UF 1:3000 n/a Rabbit pS6 (Ser235/236) Cell Signaling Technology, Inc., 2211 1:800 n/a Mouse S6 Cell Signaling Technology, Inc.; 2317 1:800 n/a Mouse HSP70 Stressgen; SPA-810 1:1000 1:250 Mouse LAMP1 Stressgen; VAM-EN001 n/a 1:250 Rabbit Cathepsin-D Cortex Biochem, San Leanardo, CA, USA; CP3090 1:1000 n/a Rabbit LC3 Cell Signaling Technology; 2775 1:1000 1:200 Open in a separate window WB: Western Blot; IS: Immunostaining; n/a: non-applicable Primary antibodies used in this study
Article Title: The molecular architecture of myelinated peripheral nerves is supported by calorie restriction with aging
Article Snippet: To determine statistical significance between samples, unpaired t-test was performed using GraphPad Prism v5.0 software.Graph pad Prism software. table ft1 table-wrap mode="anchored" t5 caption a7 Species Antigen Source and Catalog # Dilution WB IS Chicken P0 Gift from Dr.Gerry Shaw, UF 1:10000 1:500 Rabbit PMP22 Pareek et. al, 1997; n/a 1:1000 n/a Rat MBP Chemicon, Temecula; MAB386 1:5000 n/a Mouse GAPDH Encor Biotechnology Inc.; MCA-1D4 1:5000 n/a Mouse NF-H Encor Biotechnology Inc.; MCA-NAP4 1:500 n/a Chicken NF-M Encor Biotechnology Inc.; CPCA-NF-M 1:5000 n/a Chicken NF-L Encor Biotechnology Inc.; CPCA-NF-L 1:5000 1:1000 Mouse Vimentin Sigma, St Louis, MO, USA; clone VIM 13.2 1:1000 1:500 Chicken MBP Encor Biotechnology, Inc.; CPCA-MBP n/a 1:500 Rabbit p75 NTR Chemicon; AB1554 1:10000 n/a Mouse pHH3 (Ser10) Millipore, Temecula; 05–598 1:500 1:200 Rabbit Pan-Na v (α-subunit) Chemicon; AB5210 1:250 1:100 Rabbit Kv1.1 Chemicon; AB5174 1:250 1:100 Rabbit HSF1 Stressgen; Victoria British Columbia; SPA- 901 1:500 1:250 Rat HSP90 Stressgen, SPA-835 1:2000 n/a Rabbit HSP70 Stressgen; SPA-812 1:1000 1:500 Rabbit HSP40 Stressgen; SPA-400 1:2000 n/a Goat HSP27 Santa Cruz Biotechnology, Inc.; sc-1049 1:2000 n/a Rabbit αB-crystallin Stressgen; SPA-223 1:1000 n/a Rabbit LAMP1 Gift from Dr. William Dunn Jr, UF 1:2000 n/a Rabbit Atg7 Gift from Dr. William Dunn Jr, UF 1:3000 n/a Rabbit pS6 (Ser235/236) Cell Signaling Technology, Inc., 2211 1:800 n/a Mouse S6 Cell Signaling Technology, Inc.; 2317 1:800 n/a Mouse HSP70 Stressgen; SPA-810 1:1000 1:250 Mouse LAMP1 Stressgen; VAM-EN001 n/a 1:250 Rabbit Cathepsin-D Cortex Biochem, San Leanardo, CA, USA; CP3090 1:1000 n/a Rabbit LC3 Cell Signaling Technology; 2775 1:1000 1:200 Open in a separate window WB: Western Blot; IS: Immunostaining; n/a: non-applicable Primary antibodies used in this study Immunochemistry Schwann cells on glass coverslips were fixed with 4 % paraformaldehyde (10 min) and permeabilized with ice-cold 100% methanol (5 min).
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